This project generated experimental data on locomotor performance in Xenopus laevis under controlled thermal and immunological conditions. The dataset comprises measurements of locomotor endurance (total distance traveled and time spent moving until exhaustion) and jump force (maximum resultant force, in Newtons), obtained at three temperatures (15, 22, and 27 °C) and under two treatments (control and LPS-induced immune challenge). Additional variables include individual body mass, treatment group, temperature condition, and time post-injection (1 h and 24 h). The project also produced raw and processed datasets, as well as statistical analysis scripts developed in R.